Search results for "Neisseria gonorrhoeae"
showing 10 items of 12 documents
Whole-genome sequencing of Neisseria gonorrhoeae in a forensic transmission case.
2019
Abstract Molecular epidemiology and phylogenetic analyses are frequently used in the investigation of viral transmission cases in forensic contexts. Here, we present the methods and results of the analysis of a bacterial transmission episode in an alleged child abuse case using complete genome sequences obtained by high-throughput sequencing (HTS) methods. We obtained genomes of Neisseria gonorrhoeae from the victim, the suspect, and 29 unrelated controls. The analysis of the genomes revealed that the victim and suspect isolates had identical sequences in both the bacterial chromosome and the single plasmid present in them. One of the local controls was very similar (differing in only 2 SNP…
The diagnosis of chronic endometritis in infertile asymptomatic women: a comparative study of histology, microbial cultures, hysteroscopy, and molecu…
2017
Background Chronic endometritis is a persistent inflammation of the endometrial mucosa caused by bacterial pathogens such as Enterobacteriaceae, Enterococcus, Streptococcus, Staphylococcus, Mycoplasma, and Ureaplasma. Although chronic endometritis can be asymptomatic, it is found in up to 40% of infertile patients and is responsible for repeated implantation failure and recurrent miscarriage. Diagnosis of chronic endometritis is based on hysteroscopy of the uterine cavity, endometrial biopsy with plasma cells being identified histologically, while specific treatment is determined based on microbial culture. However, not all microorganisms implicated are easily or readily culturable needing …
Antibacterial Activity of Propolis Extracts from the Central Region of Romania against Neisseria gonorrhoeae
2021
(1) Background: Sexually transmitted infections (STIs) are among the most common infections worldwide, many of these being caused by Neisseria gonorrhoeae (NG). Increased antimicrobial NG resistance has been reported in recent decades, highlighting the need for new sources of natural compounds with valuable antimicrobial activity. This study aims to determine the effect of propolis extracts on NG strains, including antibiotic-resistant strains. (2) Methods: First void urine samples from presumed positive STI subjects were harvested. DNA was extracted, purified, and amplified via PCR for the simultaneous detection of 6 STIs. The presence of the dcmH, gyrA, and parC genes was checked in the D…
Neisseria gonorrhoeae antimicrobial resistance in Spain: a prospective multicentre study.
2021
Abstract Objectives Gonococcal infection is one of the most reported sexually transmitted infections and antimicrobial resistance in Neisseria gonorrhoeae (NG) is challenging for the treatment of this infection. This observational study aimed to describe antimicrobial resistance of NG and epidemiological data from patients with gonococcal infection in eight regions of Spain, for updating the local therapeutic guidelines. Methods MICs of penicillin, cefixime, ceftriaxone, azithromycin, ciprofloxacin, fosfomycin and gentamicin were determined by Etest for all NG isolates recovered from 1 April 2018 to 30 September 2019 from 10 hospitals in Spain. Resistance determinants were identified using …
Eksperimentāla modeļa izstrāde neisseria gonorrhoeae antibakteriālas rezistences noteikšnai
2019
Neisseria gonorrhoeae infekcija mūsdienās ir globāla problēma. Latvijā tā ir otrā visbiežāk izplatītā bakteriālā seksuāli transmisīvā slimība. Mūsdienās satraucošā N. gonorrhoeae rezistence pret antibiotikām tiek atzīmēta visā pasaulē, tā ir padarījusi ārstēšanu nekomplicētai gonokoku infekcijai vēl sarežģītāku, ar mazāk ārstēšanas iespējām nekā agrāk. Visu laiku ārstiem pastāv jautājums, kā efektīvāk un ar maksimālu precizitāti noteikt antibakteriālo rezistenci. Tehnoloģijas nepārtraukti attīstās, tāpēc, teorētiski, pētījumi ar silīcija dioksīda mirkodaļiņām un to mijiedarbību ar mikroorganismiem varētu būt nozīmīgi antibakteriālās rezistences noteikšanai. Darba mērķis: Eksperimentāli note…
Molecular evolution and complete genome sequences in forensic analysis: Neisseria gonorrhoeae in a transmission case
2019
Molecular epidemiology and phylogenetic analyses are frequently used in the investigation of viral transmission cases in forensic contexts. Here, we present the methods and results of the analysis of a bacterial transmission in an alleged child abuse case using complete genome sequences obtained by high-throughput sequencing (HTS) methods.
DNA Amplification Fingerprinting for Subtyping Neisseria gonorrhoeae Strains
1995
Background and Objectives DNA amplification fingerprinting is used in most epidemiologic studies as a substitute for conventional typing methods. DNA amplification fingerprinting and conventional typing methods were compared in this epidemiologic study of Neisseria gonorrhoeae. Goal of This Study To differentiate 70 Neisseria gonorrhoeae isolates from untreated patients with urogenital gonococcal infection. Study Design Gonococcal strains were characterized by auxo-typing, serotyping, plasmid profile, antibiotic sensitivity, and DNA amplification fingerprinting. The method of unweighted pair-group average linkage was used for cluster analysis. Discriminatory power was calculated applying Si…
Uroģenitālās hlamidiozes un gonorejas epidemioloģisko datu analīze Latvijā laika posmā 2008.-2013.g.
2015
Joprojām nav skaids kā labāk kontrolēt uroģenitālās hlamidiozes un gonokoku infekcijas transmisiju un novērst šo slimību komplikācijas, jo nepastāv vienotas vispārpieņemtas skrīninga programmas. Uroģenitālās hlamidiozes un gonorejas skrīninga programmas lietderīgums, tai skaitā izmaksu ziņā, ir diskutabls. Taču šo slimību diagnostika joprojām atrodas suboptimālā līmenī, tādēļ tās epidemioloģiskie dati nav visai ticami. Mērķis šīm pētījumam ir noteikt uroģenitālās hlamidiozes un gonokoku infekcijas reģistrēto gadījumu tendences atkarībā no pacientu vecuma, dzimuma un dzīvesvietas, apkopojot šo slimību epidemioloģiskus datus – saslimstību Latvijā laika periodā 2008.-2013. g. Dati pētījumam ar…
The power and limitations of genomic surveillance of bacteria.
2019
Host glycoprotein Gp96 and scavenger receptor SREC interact with PorB of disseminating Neisseria gonorrhoeae in an epithelial invasion pathway.
2007
Neisseria gonorrhoeae expresses numerous surface proteins that mediate bacterial adherence and invasion during infection. Gonococci expressing serotype A of the major outer membrane porin PorB (PorB(IA)) are frequently isolated from patients with severe disseminating infections. PorB(IA) triggers efficient adherence and invasion under low phosphate conditions mimicking systemic bloodstream infections. Here, we identify the human heat shock glycoprotein Gp96 and the scavenger receptor SREC as PorB(IA)-specific receptors. Gonococci expressing PorB(IA), but not those expressing PorB serotype B instead, bind to purified native or recombinant Gp96. Depletion of Gp96 from host cells prevented adh…